FACE Experiments at Maricopa,
Steven W. Leavitt and PIs/Collaborators over the years (Hyrum Johnson, Paul Pinter, Eldor Paul, Gary Wall, Elise Pendall, Talbot Brooks, Li Cheng, George Hendry, Bruce Kimball, Hyrum Johnson, Hugo Rodgers, Dave Williams, and many, many others…)
¯Free-Air CO2 Enrichment (FACE) experiments are now being conducted around the world. These experiments are conducted outdoors without the confining elements of indoor growth chamber and outdoor chamber experiments, thereby adding greater realism to experimental conditions. Generally there are ambient control plots with CO2 concentrations of the current atmosphere, and enriched treatments with CO2 concentrations elevated by at least 200 ppmV relative to the control. A distribution of pipes with holes facing inward to the plot is used to add CO2 with valves controlling distribution depending on wind direction.
¯At Maricopa, Arizona, FACE experiments have taken place since 1989 with enriched CO2 plots at ambient+200ppmV:
1989, 1990, 1991 Cotton (CO2 x H2O)
1992-93, 1993-94 Wheat (CO2 x H2O)
1995-96, 1996-97 Wheat (CO2 x N)
1998, 1999 Sorghum (CO2 x H2O)
¯Since 1991, we have been using isotopes in the experiment in a wide range of activities such as:
ØIsotopic consistency of source of tank CO2
ØIndependent verification of intended CO2 concentration in enriched treatments
ØAssessing variability of spatial isotopic homogeneity in enriched treatments
ØAir
ØPlants
ØWater-Use Efficiency estimation
ØHomogeneity of soil organic carbon (SOC) isotopic composition
ØC inputs to SOC pools
ØC losses from SOC pools
ØSOC isotopic signal through time
Øsoil respiration evidence
¯In
tracing carbon into soils, the isotopic composition of the tank CO2
and the plants provides a tracer to follow carbon into the soil organic carbon
(SOC) pools and out via soil respiration. In the 1999 FACE sorghum experiment,
the isotopic compositon of the plants was very 13C-enriched
relative to the SOC (d13C =
-23.5‰):
The shift in carbon isotopic composition of the SOC before and after the experiment can be used to estimate the carbon inputs:
Maricopa Isotope Publications/Presentations:
Cheng, L., Leavitt, S.W., Brooks, T. J.,
Matthias, A.D., Williams, D.G., Thompson, T.L., Kimball, B.A., Wall, G.W.,
Pinter Jr., P.J., 2002. Seasonal soil respiration and soil CO2
under elevated CO2 in a C4-sorghum agroecosystem. Ecological Society of
Cheng, L., Leavitt,
S.W., Kimball, B.A., Pinter, P.J. Jr., Ottman, M.J.,
Matthias, A., Wall, G.W., Brooks, T., Williams, D.G., and Thompson, T.L., 2007.
Dynamics of labile and recalcitrant soil carbon pools in a sorghum free-air CO2
enrichment (FACE) agroecosystem. Soil Biology and
Biogeochemistry in press.
Cheng, L., Martens,
D., Leavitt, S.W., Kimball, B.A., Matthias, A.D., Ottman,
M.J., Williams, D.G., Wall, G.W. and Pinter, P.J., Jr., 2003. Free Air-CO2 Enrichment (FACE) of C4-Sorghum:
Biochemical Composition and Decomposition of Sorghum Tissues Grown Under
Elevated CO2. ASA-CSSA-SSSA Annual Meeting,
Derner, J.D., Johnson, H.B., Kimball, B.A., Pinter, P.J. Jr., Polley, H.W., Tischler, C.R., Boutton, T.W., LaMorte, R.L., Wall, G.W., Adam, N.R., Leavitt, S.W., Ottman, M.J., Matthias, A.D. and Brooks, T.J., 2003. Above- and belowground responses of C3-C4 species mixtures to elevated CO2 and soil water availability. Global Change Biology. 9: 452-460
Johnson, H.B., Kimball, B., and
Leavitt, S., 1994. Carbon isotope
assessment of intrinsic water use efficiency of C3 plants in two
FACE experiments. Ecological Society of
Leavitt, S.W., Paul,
E.A., Kimball, B.A., Hendrey, G.R., Mauney, J.R., Rauschkolb, R.,
Rogers, Jr., H., Lewin, K.F., Nagy, J., Pinter, Jr.,
P.J. and Johnson, H.B., 1994. Carbon isotope dynamics of CO2-enriched
FACE cotton and soils. Agricultural and
Leavitt, S.W.,
Follett, R.F. and Paul, E.A., 1996.
Estimation of slow and fast-cycling organic carbon pools from 6N HCl hydrolysis. Radiocarbon
38: 231-239.
Leavitt, S.W., Paul,
E.A., Pendall, E., Pinter, P.J. and Kimball, B.A.,
1996. Field
variability of carbon isotopes in soil organic carbon. Nuclear Instruments and Methods in Physics
Research B 123: 451-454.
Leavitt, S.W., Paul,
E.A., Galadima, A., Nakayama, F.S., Danzer, S.R., Johnson, H. and Kimball, B.A., 1997. Carbon isotopes and carbon
turnover in cotton and wheat FACE experiments. Plant and Soil 187: 147-155.
Leavitt, S.W., Pendall, E., Paul, E.A., Brooks, T., Kimball, B.A., Pinter, P.J. Jr., Johnson, H.B., Matthias, A., Wall, G.W., and LaMorte, R.L., 2001. Stable-carbon isotopes and soil organic carbon in wheat under CO2 enrichment. New Phytologist 150: 305-314.
Matthias, A.D., Leavitt, S.W., Thompson, T.L., Kimball,
B.A., Pinter Jr., P.J., Wall, G.W., Rauschkolb, R.S.,
Ottman, R.L., Roth, R.L., Brooks, T.J., Adam, N.R., LaMorte, R.L., Wechsung, G., Wechsung, F., Adamsen, F.J.,
Williams, D.G., Nakayama, F.S., Hunsaker, D.J.,
Watson, J., White, S.A., and Welzmiller, J.,
2001. Free-air CO2 enrichment
effects on wheat and sorghum at
Pendall, E., Leavitt, S.W.,
Brooks, T. and Kimball, B., 1997. Enhanced soil respiration and decomposition under elevated carbon
dioxide. 7th Annual V.M.
Goldschmidt Conference, 2-6 June,
Pendall, E., Leavitt, S.W., Brooks, T., Kimball, B.A., Pinter, P.J. Jr., Wall, G.W., LaMorte, R.L., Wechsung, G., Wechsung, F., Adamsen, F., Matthias, A.D., Thompson, T.L., 2001. Elevated CO2 stimulates soil respiration in a FACE wheat field. Basic and Applied Ecology 2: 193-201.
Thompson, T.L.,
Williams, D.G., Gempko, V., Fravolini, A., Leavitt, S.W., Wall, G.W., Kimball, B.A., Pinter, P.J. Jr., LaMorte, R.L., and Ottman, M., 2001. Carbon isotope discrimination by Sorghum bicolor under CO2 enrichment and drought. New Phytologist 150: 285-293.